Immunohistochemistry of Whole-Mount Mouse Embryos
نویسندگان
چکیده
منابع مشابه
Immunohistochemistry of whole-mount mouse embryos.
INTRODUCTIONThe technique presented this protocol is ideal for obtaining an overall, three-dimensional picture of the distribution of an antigen in embryos from the prestreak stage to ~10.5 dpc (days post-coitum). The embryos are fixed and permeabilized so that antibodies can penetrate all of the tissues. However, antibodies will not penetrate the embryo completely if it is too large, and so it...
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The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sen...
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The whole-mount in situ hybridization process has revolutionized the study of gene expression in the embryo. This procedure allows extremely sensitive detection of RNA transcripts and excellent spatial resolution. Numerous experiments benefit from the detection of more than one marker molecule in the same experimental embryo. While antisense RNA probes are extremely useful and methods for two-c...
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Large-scale mouse mutagenesis experiments now under way require appropriate screening methods. An important class of potential mutants comprises those with defects in the development of normal cerebellar patterning. Cerebellar defects are likely to be identified often because they typically result in ataxia. Immunohistochemistry (IHC) is commonly used to reveal cerebellar organization. In parti...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2008
ISSN: 1559-6095
DOI: 10.1101/pdb.prot4820